INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Hydrogel Scaffolds with Immobilized Bacteria for 3D Cultures
Autor/es:
MARÍA C. GUTIÉRREZ; ZAIRA Y. GARCÍA-CARVAJAL; MATÍAS JOBBÁGY; LUIS YUSTE; FERNANDO ROJO; CONCEPCIÓN ABRUSCI; FERNANDO CATALINA; FRANCISCO DEL MONTE; MARÝÍA L. FERRER
Revista:
CHEMISTRY OF MATERIALS
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: WASHINGTON; Año: 2007 vol. 19 p. 1968 - 1968
ISSN:
0897-4756
Resumen:
We have studied the suitability of a cryogenic process (e.g., ice-segregation-induced self-assembly, ISISA) for preparation of polyvinyl alcohol (PVA) scaffolds with in situ immobilized bacteria (e.g., Escherichia coli). Confocal fluorescence microscopy and impedance measurements were used to evaluate the extension of bacteria proliferation within the scaffold macrostructure. The bacteria efficiency for colonization of the scaffold macrostructure is compared for bacteria immobilized with and without the use of additional cryoprotectans. Cryoprotection by bacteria entrapment in alginate beads containing glucose results in a significant improvement (more than 2-fold as compared to non-cryoprotected) of bacteria proliferation within the PVA scaffold. Results are also compared with the most widely used method for cells colonization of scaffolds; i.e., soaking of a preformed PVA scaffold in bacteria culture medium.