Hydrogel Scaffolds with Immobilized Bacteria for 3D Cultures

MARÍA C. GUTIÉRREZ; ZAIRA Y. GARCÍA-CARVAJAL; MATÍAS JOBBÁGY; LUIS YUSTE; FERNANDO ROJO; CONCEPCIÓN ABRUSCI; FERNANDO CATALINA; FRANCISCO DEL MONTE; MARÝÍA L. FERRER

CHEMISTRY OF MATERIALS

AMER CHEMICAL SOC

Lugar: WASHINGTON; Año: 2007 vol. 19 p. 1968 - 1973

0897-4756

We have studied the suitability of a cryogenic process (e.g., ice-segregation-induced self-assembly, ISISA) for preparation of polyvinyl alcohol (PVA) scaffolds with in situ immobilized bacteria (e.g., Escherichia coli). Confocal fluorescence microscopy and impedance measurements were used to evaluate the extension of bacteria proliferation within the scaffold macrostructure. The bacteria efficiency for colonization of the scaffold macrostructure is compared for bacteria immobilized with and without the use of additional cryoprotectans. Cryoprotection by bacteria entrapment in alginate beads containing glucose results in a significant improvement (more than 2-fold as compared to non-cryoprotected) of bacteria proliferation within the PVA scaffold. Results are also compared with the most widely used method for cells colonization of scaffolds; i.e., soaking of a preformed PVA scaffold in bacteria culture medium.