INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Time Resolved Electrochemical Quantification of Azanone (HNO) at Low Nanomolar Level.
Autor/es:
SEBASTIÁN A. SUÁREZ; DAMIAN BIKIEL; DIANA WETZLER; MARCELO MARTI; FABIO DOCTOROVICH
Revista:
ANALYTICAL CHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2013 vol. 85 p. 10262 - 10269
ISSN:
0003-2700
Resumen:
Azanone (HNO, nitroxyl) is a highly reactive
and short-lived compound with intriguing and highly relevant
properties. It has been proposed to be a reaction intermediate
in several chemical reactions and an in vivo, endogenously
produced key metabolite and/or signaling molecule. In
addition, its donors have important pharmacological properties.
Therefore, given its relevance and elusive nature (it reacts
with itself very quickly), the development of reliable analytical
methods for quantitative HNO detection is in high demand for
the advancement of future research in this area. During the
past few years, several methods were developed that rely on
chemical reactions followed by mass spectrometry, high-performance liquid chromatography, UV−vis, or fluorescence-trappingbased
methodologies. In this work, our recently developed HNO-sensing electrode, based on the covalent attachment of
cobalt(II) 5,10,15,20-tetrakis[3-(p-acetylthiopropoxy)phenyl] porphyrin [Co(P)] to a gold electrode, has been thoroughly
characterized in terms of sensibility, accuracy, time-resolved detection, and compatibility with complex biologically compatible
media. Our results show that the Co(P) electrode: (i) allows time-resolved detection and kinetic analysis of the electrode
response (the underlying HNO-producing reactions can be characterized) (ii) is able to selectively detect and reliably quantify
HNO in the 1−1000 nM range, and (iii) has good biological media compatibility (including cell culture), displaying a lack of
spurious signals due to the presence of O2, NO, and other reactive nitrogen and oxygen species. In summary, the Co(P)
electrode is to our knowledge the best prospect for use in studies investigating HNO-related chemical and biological reactions