Influence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study

RENATO APARECIDO DE SOUZA; MURILO XAVIER; FABIANO FERNANDES DA SILVA; MARCO TÚLIO DE SOUZA; MAIRA GASPAR TOSATO; AIRTON ABRAHÃO MARTIN; JULIO CEZAR DE MELO CASTILHO; WELLINGTON RIBEIRO; LANDULFO SILVEIRA JR

LASERS IN MEDICAL SCIENCE

SPRINGER LONDON LTD

Lugar: London; Año: 2012 vol. 27 p. 487 - 495

0268-8921

The influence of creatine (Cr) supplementation on cortical and trabecular bone from ovariectomized rats was studied using FT-Raman spectroscopy. The intensity of organic-phase Raman bands was compared to mineral phase ones. Twenty-one female Wistar rats aged 3 months were divided into three groups (n = 7 per group): ovariectomized (OVX), ovariectomized treated with creatine (CRE) and sham-operated (SHAM) groups. Creatine supplementation (300 mg kg−1 day−1) was provided for 8 weeks, starting 12 weeks after ovariectomy. FT-Raman spectroscopy was performed on the right medial femoral mid-shaft (cortical bone) and third lumbar vertebral body (trabecular bone). The integrated intensities of mineral phase (phosphate and carbonate bands at 959 and 1,071 cm−1, respectively) and organic phase (amide I band at 1,665 cm−1) Raman bands were analyzed. The mineral-to-matrix (phosphate/amide I), carbonate-to-phosphate, and carbonate-to-amide I ratios were analyzed to assess bone quality. The phosphate content on trabecular bone was higher in the CRE group than the OVX group (p