INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Development of a Biosensor Based on Immobilized Microalgae in Silica Hydrogels for Environmental Monitoring
FERRO, YANNIS; MERCEDES PERULLINI; JOBBAGY, MATIAS; SARA A. BILMES; CLAUDE DURRIEU
MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI
Lugar: Basel; Año: 2012 vol. 12 p. 16879 - 16879
A new biosensor is designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two steps procedure. After studied growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to DCMU and various concentrations of Atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement was realized with very good efficiency. The best detection limit was 0.1 µM, obtained with the strain C. reinhardtii after 40 minutes of exposure.