INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Structure and Thickness Dependence of "Molecular Wiring" in Nanostructured Enzyme Multilayers
Autor/es:
ERNESTO JULIO CALVO; VICTORIA FLEXER; SILVIO LUDUENA; ERICA FORZANI; LIA PIETRASANTA
Revista:
ANALYTICAL CHEMISTRY
Editorial:
American Chemical Society
Referencias:
Año: 2006 vol. 78 p. 399 - 399
ISSN:
0003-2700
Resumen:
Supramolecular organized multilayers composed of glucose oxidase (GOx) and osmium-derivatized poly(allylamine) redox polymer have been self-assembled electrostatically from Os-polyelectrolyte solutions of variable pH (5.5-8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. (5.5-8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. -polyelectrolyte solutions of variable pH (5.5-8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. -8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-bylayer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. 2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of â-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change in the film growth mechanism is observed. rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. where a change