INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
2. Detection of low quantum yield fluorophores with improved imaging time using metallic nanoparticles.
ESTRADA LC, ROBERTI MJ, SIMONCELLI S, LEVI V, ARAMENDÍA PF, MARTÍNEZ OE
JOURNAL OF PHYSICAL CHEMISTRY B
Washington, D.C. : American Chemical Society
Año: 2012 vol. 116 p. 2306 - 2306
The behavior of a fluorophore near a gold nanoparticle8 is rationalized by a theoretical description of the parameters that9 modify the fluorescence emission: nanoparticle−fluorophore distance,10 fluorescence quantum yield (ϕ0), and fluorophore absorption and11 emission spectra, to find optimum conditions for designing12 fluorophore−nanoparticle probes. The theoretical maximum gain in13 brightness of the nanoparticle−fluorophore system with respect to the14 isolated molecule increases almost inversely proportional to ϕ0. The15 brightness enhancement in imaging experiments in vitro was assessed16 by using Au-SiO2 core−shell nanoparticles deposited on glass. A ∼13-fold emission brightness enhancement for weakly17 fluorescent molecules was observed. A significant increase in fluorophore photostability, rendering longer imaging times, was18 obtained for fluorophores interacting with gold nanoparticles incorporated by endocytosis in cells. Our results illustrate a way to19 increase imaging times and to study molecules in the vicinity of a metallic nanoparticle after photobleaching of background20 fluorescence.