INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
Autor/es:
MERCEDES PERULLINI; MAKHLOUF AMOURA; MATÍAS JOBBÁGY; CÉCILE ROUX; JACQUES LIVAGE; THIBAUD CORADIN; SARA A. BILMES
Revista:
JOURNAL OF MATERIALS CHEMISTRY
Editorial:
ROYAL SOC CHEMISTRY
Referencias:
Año: 2011 vol. 21 p. 8026 - 8026
ISSN:
0959-9428
Resumen:
A two-step encapsulation process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles.  In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species suggest that both forms of Zr may be involved in the cytotoxicity of the precursor solution. In addition, this optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide-alginate hybrid gel. These data suggest that sol-gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.