INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of Arabidopsis mutants deficient in alkaline-neutral invertases
Autor/es:
ADRIANA QUIÑONES; STEFANIA COHEN; GRACIELA SALERNO; EDUARDO ZABALETA; MARIANA MARTIN
Lugar:
Puerto Madryn
Reunión:
Congreso; 46 Reunión Anual de la SAIB; 2010
Institución organizadora:
CONICET-ANPCyT-EMBO-IUBMB-ALUAR S.A.I.C.
Resumen:
Invertases (Invs) catalyze the irreversible hydrolysis of sucrose into hexoses and play an important role when a demand of carbon and energy occur. Moreover, it has become evident that sucrose and its hydrolysis products are important metabolic signals that modulate gene expression and regulate plant development. Alkaline-neutral invertases (A/N-Invs) are one of the two classes of invertase activities with a characteristic optimum pH between 6.5–8.0. The physiological functions of A/N-Invs are not clear yet. A/N-Invs are distributed between the cytosol and different subcellular locations suggesting different physiological functions. In this work, we focus on two potentially mitochondrial A/N-Invs in Arabidopsis (At-INVA and At-INVC). To explore their possible biological roles we analyzed At-a/n-inva and At-a/n-invc knockout mutant plants. Lack of At-A/N-INVA and At-A/N-INVC expression in homozygous mutant plants was verified by RT-PCR assays. Phenotype characteristics were analyzed for both mutants. Moreover, knockout plants display a lower total A/N-Inv activity compared to wild-type plants. Possible functional roles of alkaline/neutral invertases in mitochondria will be discussed.