Molecular analyses of Fusarium poae based on nuclear and mitochondrial sequences

S.A. STENGLEIN; M.S. RODRIGUERO; E. CHANDLER; G.L. SALERNO; P. NICHOLSON

Huerta Grande, Córdoba

Jornada; XXXII Jornadas Argentinas de Botánica; 2009

A molecular phylogenetic analysis of Fusarium poae isolates from South America (Argentina) and Europe (mainly England, Germany, Italy) was performed using 98 F. poae, 3 F. avenaceum, 3 F. tricinctum, 4 F. culmorum, 2 F. sporotrichioides and 1 F. langsethiae isolates. Phylogenetic analyses of the nuclear (translation elongation factor 1-alpha, EF-1a) and mitochondrial (mitochondrial small subunit rDNA, mtSSU) sequences were performed using Maximum Parsimony and Bayesian Inference. The EF-1a dataset consisted of 711 nucleotide characters, of which 156 were parsimony informative. The total proportions of nucleotides were 22.9% A, 29.2% C, 21.6% G, 26.3% T. Sequences comprising the 667 bp mtSSU dataset contained 45 parsimony informative characters. In this case, the total proportions of nucleotides were 32.9% A, 15.2% C, 24.1% G, 27.9% T. The consistency and retention indexes were 0.99 for EF-1a and 1 for mtSSU. Molecular diversity values indicated a lower level of genetic variation for the mtSSU than the EF-1a dataset. Individual and total evidence analyses did not reveal any clear correlations, from the inferred branching topology, between the distribution of observed haplotypes and the geographic origin and/or host species.