Characterization of new Glu-3 alleles

IKEDA, T.M.; BRANLARD, G.; PEÑA, R.J.; TAKATA, K.; LIU, L.; HE, Z.H.; LERNER, S.E.; KOLMAN, M.A.; YOSHIDA, H.; ROGERS, W.J.

Clermont Ferrand, France

Workshop; Xth International Gluten Workshop; 2009

International Gluten Workshop

Low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat is one of the critical determinants of gluten properties.  However, identification of Glu-3 alleles is difficult, due to the complexity of the LMW-GSs.  To unify Glu-3 nomenclature for facilitation to share information about the effects of individual LMW-GS on gluten properties, we shared 107 cultivars having various Glu-A3, Glu-B3 and Glu-D3 alleles from Argentina, China, France, Japan and Mexico.  Using 2D analysis, the N-terminal amino acid sequencing and gene analysis, we found new Glu-3 alleles among these materials.  For Glu-B3, we found an additional LMW-GS in cultivars having Glu-B3b, Glu-B3g and Glu-B3i in common.  The N-terminal sequence of this LMW-GS was METSQIPGLEKP, which was not reported previously.  We developed a specific-primer set to detect this allele by PCR.  We temporally assigned these alleles to Glu-B3ab, Glu-B3ac and Glu-B3ad, respectively.  We also found two new Glu-D3 alleles.  These alleles were characterized by the absence of different two LMW-GSs.  We identify these alleles by the absence of specific PCR products corresponding to the LMW-GSs.  These results showed that a combination of protein and gene analyses is important to identify new Glu-3 alleles.