Chromatographically Isolated Exosomes From Mesenchymal Stem Cells (Mscs) Over-Expressing Heme Oxygenase-1 For Tissue Regeneration

NATALIA ALEJANDRA PACIENZA; DARWIN PROCKOP; DIEGO MARIO SANTA CRUZ ; RICARDO MALVICINI; DONG-KI KIM; GUSTAVO GABRIEL YANNARELLI

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

Regenerative properties of mesenchymal stem cells (MSCs) are largely mediated by paracrine mechanisms, including the secretion of extracellular vesicles (EVs). Exosomes are EVs of endosomal origin that may constitute a safe and effective cell- free therapy. We developed a scalable chromatographic procedure for production of exosomes derived from human MSCs over-expressing heme oxygenase-1 (HO-1) that might have anti-inflammatory immunosuppressive potential. MSCs were activated with hemin, an HO-1 inducer, for 24 h and subsequently cultured in a chemically defined protein-free media (CDPF) during 48 h to collect the exosomes. Hemin treatment up-regulated HO-1 mRNA expression by 54±14-fold compared with control MSCs (p