INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CONTROL OF CHARCOAL ROT DISEASE BY PGPR STRAINS: THE ROLE OF ANTIOXIDANT SYSTEM IN SOYBEAN PLANTS
Autor/es:
CARLA ZILLI; SANTA-CRUZ DM; SIMONETTI E; HERNANDEZ A; CARMONA M; BALESTRASSE KB
Reunión:
Congreso; 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015
Resumen:
Charcoal rot is a plant disease caused by the soil fungus Macrophomina phaseolina. The biocontrol agents 9 and 54were tested for their effectiveness against root rot of soybean caused by Macrophomina phaseolina. Both PGPRstrains were isolated from disease suppressive soils in different locations of Santa Fe Province. The fungal strain wasisolated from infected soybean plants showing root rot symptoms. The control of disease was determined accordingto the ability to avoid fungal colonization. Inoculation with strain 9 was the most effective treatment to controlinfection (54%).In situ production of ROS was evaluated by Schiff, DAB and NBT staining. Rootlets infectedshowed a 2.3-fold increase on HO levels. Strains 9 and 54 improved partially HO content. None of the appliedtreatments was able to reverse the suppressive effect of the pathogen on Oproduction, while gene expressionantioxidant enzyme was downregulated in infected radicles. However, as a result of strain 9-fungus interaction, SOD,CAT, APOX and HO genes were overexpressed. In conclusion,fungal colonization was efficiently prevented byinoculation with strain 9. Its ability to improve ROS production and gene expression of antioxidant enzymes could berelated to its capacity to control disease