134. Fusarium poae mitochondrial samll subunit rDNA sequences present an IC2 intron

S.A. STENGLEIN; E. CHANDLER; P. NICHOLSON; M.V. MORENO; G.L. SALERNO

Mar del Plata, Prov. de Buenos Aires

Congreso; VI CLAM. Congreso Latinoamericano de Micología; 2008

Asociación Latinomaricana de Micología

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES; mso-fareast-language:ES;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Among Fusarium species, F. poae is one of the FHB and mycotoxins producer species. In a phylogenetic study based on mtSSU rDNA and EF-1α sequences obtained from 48 Argentinean and 50 European strains, we found that 9 Argentinean F. poae strains from Argentina present an insert of ±1600 bp in their mtSSU sequences. A BLASTx search using public databases and the insert sequence as query showed an E value=2 e-74 and 6 e-72 with LAGLIDAG endonuclease from Podospora anserine and Gibberella zeae, respectively. Further bioinformatic analyses using the Rfam and RNAweasel programs revealed that the insert sequence arranged with the group I catalytic intron IC2. The presence of this intron in the mtSSU sequence may lead to interesting evolutionary conclusions within Argentinean F. poae strains. This is the first report on the existence of introns in the mtSSU sequence of F. poae.