INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fluorescence in root exudates of soybean seedling is influenced by phosphate starvation.
Autor/es:
YARYURA, P., M LEON, G CORDON, M. G LAGORIO, N KERBER, N PUCHEU, A GARCÍA, G RUBIO.
Lugar:
Mar del Plata
Reunión:
Congreso; XLII Reunión Sociedad Argentina de Investigación bioquímica y Biología Molecular (SAIB).; 2007
Institución organizadora:
SAIB
Resumen:
Phosphorus is one of the essential macronutrients in plants. Phosphate starvation leads to changes in morphological, physiological and biochemical processes. Some roots fluoresce when irradiated with UV light. This trait has been useful as a marker in genetic studies. We studied the influence of phosphate starvation on the amount of root and seed exudates and leaves fluorescence in two soybean genotypes (FN-4.85 and FN-4.10). Plants were grown in hydroponic cultures. The fluorescence was determined with a spectrofluorometer in 21 days-old plants. Our results indicated that fluorescence emission (F420nm) of root exudates was significantly higher (p<0.05) in FN 4.85 and significantly lower in FN 410 (p<0.05) in phosphate deficient plants. In seeds, fluorescence had a different maximum emission band (F503nm) than root exudates, but was identical between both genotypes. Fluorescence in leaves showed the same emission spectrum (F420nm) of root exudates, but no effects of phosphate limitation was detected. We also determined that the fluorescence ratio (F684/F737) for chlorophyll emission did not vary under phosphate starvation. This is an indication that growth for 21 days under the latter condition had not yet induced quali/quantitative changes in photosystems. These results could reflect the genetic variability in soybean which could perhaps be extended to other soybean genotypes.