INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
OXIDATIVE RESPONSE IN SOYBEAN SEEDS AGAINST SOUTHERN STINK BUG ATTACK
Autor/es:
SABLJIC, I; BALESTRASSE KB; PAGANO E
Lugar:
Rosario
Reunión:
Congreso; SAIB 2014; 2014
Resumen:
Southern stink bug [Nezara viridula (L.) (Hemiptera: Pentatomidae)], is a major pest of soybean in countries such as Brazil, USA and Argentina where this crop is grown. The insect attack decreases yield and affects seed quality by reducing viability and vigor. It has been establish that as a consequence of this insect action, an alterartion in antioxidant enzyme activities occurs in soybean. The potential role of these enzymes is linked to signaling, synthesis of defense compounds and oxidative stress tolerance. The aim of this study was to compare the oxidative response of two contrasting genotypes of soybean against Nezara viridula attack: IAC-100 (resistant) and Davis (susceptible). Guaiacol peroxidase (GPOX), catalase (CAT) and superoxide dismutase (SOD) activities were spectrophotometrically determined in seed extracts of soybean plants grown in a greenhouse with and without treatment of stink bugs in R6 for 72 hours. To analyze H2O2 generation, seeds were excised and immersed in a 1% solution of 3,3-Diaminobenzidine (DAB). Results here presented indicate that after insect the resistant genotype has greater GPOX, CAT and SOD activities and a decreased content of peroxide indicating that there is a differential oxidative response among soybean genotypes with contrasting response to the stink bug attack.