INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation and characterization of bacterial strains from soybean rhizosphere as biopesticides
Autor/es:
SIMONETTI E; MONTECCHIA MS; PIN VISO N; ZILLI C; BALESTRASSE K
Lugar:
Buenos Aires
Reunión:
Simposio; 32nd New Phytologist Symposium: Plant interactions with other organisms: molecules, ecology and evolution; 2013
Resumen:
One novel strategy to control plant diseases is the use of antagonistic rhizobacteria. The aim of the present study is to isolate, identify and to select indigenous bacterial strains with antifungal activity from soybean plants growing in soils with disease-suppressive attributes. A total of 27 bacterial strains were obtained in a preliminary screening, from the antagonism test plates where the confluent growth of bacteria inhibited the development of fungal mycelia and microesclerotia of Macrophomina phaseolina (Tassi) Goid. In search of efficient bacterial strains with multiple activities, a total of 6 bacterial isolates were characterized based on their morphological and biochemical characters like production of indoleacetic acid, siderophore, phosphate solubilization and identified by 16S rDNA sequences as Stenotrophomonas sp., fluorescent Pseudomonas, Chryseobacterium sp. and Bacillus sp. Oxidative stress parameters were estimated in extracts of radicles emerged from seeds that were treated with the rhizobacteria and inoculated with M. phaseolina. The results suggested that the damage severity on bacterized seeds was significantly reduced compared to non-bacterized control seeds. The protective action against the pathogen was accompanied by an induction of antioxidant defenses. The rhizobacteria isolated in this work can be a useful tool to reduce charcoal root rot in soybean.