INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HEME OXYGENASE-1 AND ABSCISIC ACID AFFECT MAPK’SGENEEXPRESSIONINSOYBEANSEEDS
Autor/es:
GIACOMETTI R; SANTA CRUZ DM; NORIEGA GO; BALESTRASSE KB
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2012
Resumen:
In soybean previous studies enabled the identification of MAPK3and 6 whose activity is enhanced within the signaling pathwayleading to defense reactions. In this study the effects of differentcompounds related to hemeoxygenase (HO-1) biosynthesis onmitogen-activated protein kinase (MAPK’s) genes expression insoybean seeds were tested. To this end, 20μM hemine, 22μMZnPPIX, 0.5mM furidine or 100μM 8-bromoguanosine 3´,5´-cyclicmonophosphate (8Br) were added to pre-hydrated seeds for 5 days.MAPK’s genes expression was enhanced in seeds treated withhemine. This result indicates that heme catabolism could beinvolved in the signaling mediated by this cascade pathway. Toconfirm this hypothesis experiments were carried out in thepresence of ZnPPIX, a potent irreversible HO-1 inhibitor. In thiscase, no gene induction was observed. On the other hand, 8Br, acGMPanalog, induced HO-1 gene expression but did not modulateMAPK’s, indicating that this effect could not be mediated bycGMP. When the action of furidine, an abscisic acid inhibitor, wastested a diminution of HO-1 gene expression was observed. In thisregard, MAPK’s showed a different response, being MAPK6 theonly transcript that showed a diminished respect to controls, whileMAPK3 mRNAas well as MAPKK1 was enhanced. These resultswere confirmed by western blotting and activity determinations.