INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of a Bacillus thuringiensis strain native from Argentina toxic against mosquito species
Autor/es:
BERÓN CM; VIDAL DOMÍNGUEZ ME; DÍAZ NIETO LM
Lugar:
Buenos Aires
Reunión:
Congreso; International Congress on Invertebrate Pathology and Microbial Control and 45th Annual Meeting of the Society for Invertebrate Pathology; 2012
Institución organizadora:
SIP
Resumen:
Mosquitoes (Culicidae) are blood-sucking insects of public health importance, mainly because of their importance as vectors of many hazardous diseases, like dengue, yellow fever, malaria, several types of encephalitis including West Nile fever, and lymphatic filariasis. The application of different strategies to management of mosquito populations is essential for the control of these viral diseases. In a previous work, we characterized a Bacillus thuringiensis isolate native to Argentina (FCC 41) that exhibits mosquitocidal activity and harbourgs he protein Cry24Ca in its parasporal body. Here we describe the presence of an Orf2-like sequence downstream the secuence cry24Ca. On the other hand, we detected a new protein, Cry 50-like by biochemicals techniques like sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE) and MALDI-TOF mass spectrometry. We used a PCR-based strategy for amplification of DNA fragments with degenerated oligonucleotides homologous to cry50-like genes, followed by Tail-PCR methodology, in order to clone and sequence the entire gene. BLASTp analysis indicated that the new sequence is related to Cry 52 protein type. Moreover, in order to extend the toxicological spectra of FCC 41 strain, toxic against Aedes aegypti mosquito, we performed bioassays against other three mosquito species: Culex pipiens, Cx. apicinus and Ochlerotatus albisfaciatus. A high larvicidal activity was observed in all cases. Thus, our findings expanded the toxicological spectra of this native strain and suggest that it could be used as a microbial insecticide for the control of several vector mosquitoes species.