INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF CROP ROTATION UNDER ZERO TILLAGE ON SOIL FUSARIUM POPULATION
Autor/es:
SILVESTRO L.B., STENGLEIN S.A., FORJAN H., DINOLFO M.I., ARAMBARRI A.M., BERON C., MORENO M.V.
Reunión:
Congreso; VIII Congreso de Microbiologia General; 2012
Resumen:
The presence of Fusarium species in cultivated soils is commonly associated with plant debris and plant roots, and also associated as soil saprophytes. This ability can be attributed to the capacity of Fusarium species to grow on a wide range of substrates and their efficient dispersion mechanisms. The aim of this study was to examine the diversity of soil Fusarium spp. under different crop rotations and depths, in systems under zero tillage. Soil samples were obtained from the Barrow Experimental Station, Tres Arroyos, Buenos Aires Province. The Fusarium species were identified by morphological characteristics and by using PCR reactions with species-specific primers. For F. solani confirmation, a part of the elongation factor 1-á (EF-1á) gene was amplified, purified and sequenced. One hundred twenty two isolates of Fusarium were obtained: F. equiseti, F. merismoides, F. oxysporum, F. scirpi, and F. solani. The most prevalent species was F. oxysporum, wich was observed in all sequences and depths. The Tukey?s test showed that the relative frequency of F. oxysporum under intensive agricultural management was higher than in mixed traditional ones. The first 5 cm of soil showed statistically significant differences respect to 5-10 cm and 10-20 cm depths. The ANOVA test for the relative frequency of F. equiseti, F. merismoides, F. scirpi and F. solani, did not show statistically significant differences. We not found significant differences in the effect of crop rotations and depth on Shannon, Simpson and species richness. Therefore we conclude that the type of crop rotation and the sampling depth did not affect the alpha diversity of the Fusarium community in this system.