INBA   12521
INSTITUTO DE INVESTIGACIONES EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Protection of oilseed rape (B. napus) toward fungal pathogens by strains of biocontrol PGPR
Autor/es:
SIMONETTI, ESTER; HERNÁNDEZ, ALEJANDRA I.; KERBER, NORMA L. ; PUCHEU, NORMA L. ; GARCÍA, AUGUSTO F.
Lugar:
Puerto Madryn, Chubut República Argentina
Reunión:
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
SAIB
Resumen:
The aim of this work is to evaluate the effects of treatment of oilseed rape (Brassica napus) with indigenous bacterial strains isolated from soybean rhizosphere. Antifungal activities of two of these isolates, previously identified and designated as Pseudomonas  fluorescens BNM296 and Bacillus amyloliquefaciens BNM340, were assayed by dual culture with two serious fungal pathogens to Brassica species, Botrytis cinerea and Sclerotinia sclerotiorum. Oilseed rape seeds were inoculated with each of the bacterial suspensions and colonization of seedling roots was evaluated. In addition, the treated plants were assessed for their resistance to the fungal pathogens using detached leaves assays. The dual bacterial culture of BNM340 inhibited the myceliar growth of B. cinerea (66.4±1%) and S. sclerotiorum (71.1±5.2%) with respect to each of the fungi growing alone. In the case of BNM296, the relative growth of B. cinerea and S. sclerotiorum, in the presence of the bacteria, were also inhibited 46.1±4.6% and 37.6±6%, respectively. The quantification of bacteria adhering to the surface of inoculated B. napus seeds and roots showed that both of tested bacteria effectively colonized them. Leaves of treated plants with BNM296 and BNM340 exhibited an enhanced state of resistance against S. sclerotiorum and B. cinerea, demonstrating that these bacterial suspensions are capable of inducing ISR.