0810-Treatment of irradiated mice with oral radioprotector attenuates insult.

LUCIA CERVANTES; FERNANDA CANTERO; MARTINEZ MARIGNAC VERONICA L; GLORIA OERTLIN; FAVANT JOSE LUIS; LEONEL MONDRAGON; SILVIA VIALE

MAR DEL PLATA

Congreso; REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIONES CLINICAS; 2019

SAIC

Ionizing radiation directly affects DNA structure by inducing primarily DNA double strand breaks (DSBs), and secondarily production of reactive oxygen species (ROS) that oxidize proteins and lipids, and also induce several different damages to DNA, like generation of abasic sites and single strand breaks (SSB). Consequently, all these changes induce cell death and mitotic failure. The important use of IR in X ray exams and in radiotherapy and its undesirable effects took us to validate a murine model in order to evaluate DNA damage of X Rays and characterize natural and food supplements compounds with radio mitigation properties.Essiac Genuine tea has been used widely in the homeopathy market as a popular anticancer and antioxidant tonic. Due to the reported ROS scavenging properties of Essiac formula, we evaluate DNA damaged mitigation in 50 male Balb/c mice under 25-100mSv-Gy, which is an average effective dose given in most X Ray exams. The tea formula resulted in a significant reduction of DNA damaged of mice under the formula evidenced by Comet Assay as well as in a normalization of the complete blood count (CBC). The tea did not show any cytotoxicity at the used doses. We not only demonstrated that Essiac tea is not toxic and acts as a radioprotector of IR X rays at doses use in image exams though we also optimized a murine model for further analysis of other natural compounds and supplements.