COMPARATION OF CONTENT BETWEEN Paspalum dilatatum Poir. WILDTYPE AND COLD RESISTANCE TRANSGENIC

MARIA GABRIELA FERNANDEZ PEPI; E.S. VIÑAS; VALENTIN FADEL; ALEJANDRO ZUCOL; G. E. SCHRAUF; GUSTAVO JAURENA

Buenos Aires

Simposio; 5th International Symposium of Forage Breeding; 2015

Fac. de Agronomia UBA (FAUBA)

The summer forage Paspalum dilatatum Poir. ("pasto miel") is a native species with highproductive value, whose apomictic reproductive system confers advantages for transgenesis.Under abiotic stress were observed modified of anatomical features associated with responsetolerance to that change. Silica accumulation in plant tissues is a strategy of certain species, asa defense mechanism against herbivore and fungal attacks or enhancing the plant to toleranceto abiotic stress. It also represents a component that gives support to the leaves and stems. Theobjective of this communication was to compare qualitative and quantitative deposition of silicaand morphotypes present in the wild-type and cold-resistant transgenic plants. Five samples ofleaves and inflorescences of the two types of plant leaves were taken, and phytoliths wereobtained by calcined Labouriau´s technique, afterwards the concentration of silicates calcined indry matter was calculated. Biomineralization rates in all organs of the transgenic plants werelower than in the wild-type material. In wild plants, articulated phytoliths derived from epidermisformed by long cells and stomatal complex cross-shaped dumbbell predominated; while in thematerial from transgenic phytolith of not articulated types predominated, long prismatic anddumbbell. These first results allowed us to identify differential patterns in both the silica depositand the types of morphotypes associated with genetic modification of P. dilatatum.