CICYTTP   12500
CENTRO DE INVESTIGACION CIENTIFICA Y DE TRANSFERENCIA TECNOLOGICA A LA PRODUCCION
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ATTRACTION OF TRIATOMINES TOWARDS DIFFERENT CO2-FREE, SYNTHETIC HOST-ODOR BLENDS
Autor/es:
GUIDOBALDI F; GUERENSTEIN PG
Lugar:
Natal
Reunión:
Congreso; VIII Encontro Brasileiro de Ecologia Química; 2013
Resumen:
Vector
control is the most effective method to prevent Chagas disease. A lured trap
device is a sustainable and an environmentally-friendly tool for vector monitoring
and control. Using one of the most
important Chagas disease vectors, Rhodnius prolixus, we test potential
odor attractants for the bugs in a dual-choice olfactometer in which attracted
bugs get trapped. We already showed that a commercial, CO2-free synthetic host-odor lure
developed for mosquitoes (Sweetscent®), consisting on ammonia,
L(+)-lactic acid and hexanoic acid, evokes significant attraction and trapping
of triatomines in the laboratory. We also showed that the
insects respond specifically to the three-component blend in a synergistic way.
However, our results also suggested that the performance of the odor
blend could be improved. Thus, we now tested different variants of a blend
consisting on ammonium hydroxide, L(+)-lactic acid and pentanoic acid (blends
differed in the total mass of compounds and in their proportions). Each odor
was loaded onto an individual filter paper strip and the three strips were
placed in the test arm of the olfactometer. The negative-control arm contained
filter paper strips with the solvent (water) only. In negative-control
experiments both arms consisted in control arms. The percentage of total capture
(%TC) was defined as the total number of insects in the arms (test + control)
over the total of insects tested. Thus, we compared the odor-induced %TCs with
those of the basal %TCs obtained in the negative-control experiments. Whereas
in negative-control experiments the %TC was 7.5 % (N=53), in the positive
control (a mouse) 68.1 %TC was obtained (P<0.001, G-test, N=50). Two of the synthetic blends tested evoked
significant %TC (Blend 4: 28.1 %, G-test, P<0.01; Blend 6: 31.3 %, G-test,
P<0.01; N=32 for all six blends tested). Results indicate that, as expected,
the proportion at which the odors were delivered has a major role in
determining the attraction and capture level.
They also suggest that blends 4 and 6 are good attractants.