CICYTTP   12500
CENTRO DE INVESTIGACION CIENTIFICA Y DE TRANSFERENCIA TECNOLOGICA A LA PRODUCCION
Unidad Ejecutora - UE
artículos
Título:
3D BIOCOMPATIBLE MATRIX FOR THE BIOCHEMICAL ANALYSIS OF COMMERCIAL CANCER CELL LINES AND THEIR METABOLISM REWIRING IN RESPONSES TO THERAPEUTIC DRUGS.
Autor/es:
NATALIA ROMERO; RIDEL ERNESTO; MARTINEZ MARIGNAC VERONICA L; MOTOSLAVSKY GUSTAVO
Revista:
MEDICINA (BUENOS AIRES)
Editorial:
MEDICINA (BUENOS AIRES)
Referencias:
Lugar: Buenos Aires; Año: 2018 vol. 78 p. 238 - 238
ISSN:
0025-7680
Resumen:
One of 6 deaths worldwide is cause by cancer. The early diagnosesand treatment resulted in decreases in some countries however thestatistic worldwide is maintained. Drug screening has been one ofthe few tools with success. Generally preclinical studies use 2D cellcultures as the first strategy in the screening of compound for cancertherapy however cancer research approaches need improvementby models which could create the biological and natural architectureof the tumours; 3D cell culture is a promising approach when theyemulate natural tumour environment.Here we implement a 3D biocompatible printed matrix as commercialcell lines support for drug testing.We evaluate a colorectalcancer cell line (HCT116) and kidney embryonic cancer cell line(HEK293) as cell laden on 3D printed matrix by a 4th GenerationEnvisiontec Bioplotter. We compared drug response on 2D and 3Dmodels. 2 drugs were tested on cell lines (chloroquina, and lithiumchloride) by cytotoxicity assay (Sulforhodamine B -SRB assay) andLive/dead viability assay on 3D constructs. Our results showed inaddition to different architectural organization in 3D matrix for thecell lines , drug response differences between type of cultures, forlithium chloride HEK293 was more than 8 fold IC50 more resistant in3D culture than in 2D cultures (p