Cell laden 3D print scaffold for drug testing

MOTOSLAVSKY GUSTAVO; NATALIA ROMERO; RIDEL ERNESTO; MARTINEZ MARIGNAC VERONICA

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2018 vol. 78 p. 173 - 173

0025-7680

Traditional synthetic substrates and matrices for cell culture haveproven to be of only limited utility in efforts to understand and controlcell behavior, in large part because they fail to capture biochemical,mechanical, and dynamic characteristics of in vivo environments.However, recent advances in materials chemistry and engineeringhave provide a toolbox to mimic the complex characteristics of naturalextracellular matrices, providing new pathways to explore cell?matrix interactions and direct cell fate under physiologically realisticconditions. Here we perform a morphological and biocompatibilityanalysis of materials as dynamic substrates and matrices for 3D cellculture, and highlight their use in furthering our understanding ofhow cells respond to temporal variations in their environment. Wecompared a commercial bioink and gel combination of an in housematrix (Alginate, gelatin and pluronic F127 as sacrificial material).We used temperature and Calcium chloride as crosslinkers. Thecell ladens were HEK293 and HCT116 commercial cell lines andwe used the Envision Bioplotter to print a cylinder structures withoutinner structure on a 6 well plate from bpl file. We performed materialdegradation studies and biocompatibility. Regarding the stiffness ofthe structure we employed the range of gut tissue stiffness. The biocompatibilitywas tested by live/dead cell viability assay (2 μM calcein-acetoxymethyland 4.5 μM propidium iodide solution). Our resultsshowed that degradability and lasting of the cylinder structure madeby in house material was significantly better than the commercialbioink (p=0.04), lasting for 21 days and allowing cell survival andwhat it seems to be cell proliferation. We consider that the in house3D cell laden bioinks made of alginate gives sufficient architecturaland life support to study cell-cell interactions, and drug testing as itallow to biochemical, mechanical, geometric and dynamic characteristicsof in vivo environments to be implemented.