CICYTTP   12500
CENTRO DE INVESTIGACION CIENTIFICA Y DE TRANSFERENCIA TECNOLOGICA A LA PRODUCCION
Unidad Ejecutora - UE
artículos
Título:
Effects of sex-sorted spermatozoa on the efficiency of in vitro fertilization and ultrastructure of in vitro produced bovine blastocysts
Autor/es:
PALMA GA, OLIVIER NS, NEUMÜLLER CH, SINOWATZ F
Revista:
ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C
Editorial:
Blackwell
Referencias:
Lugar: Londres; Año: 2008 vol. 37 p. 67 - 73
ISSN:
0340-2096
Resumen:
Frozen-thawed sexed semen from six bulls (Holstein) was used for
studying their efficiency in an in vitro fertilization (IVF)-programme
and to compare their ultrastructure with in vitro produced bovine
blastocysts produced with non-sorted sperm. Progressive motility of
sorted spermatozoa, their IVF rate, development of produced blastocysts
and the ultrastructure of the blastocysts were analysed. The cleavage
rates of sexed sperm of bulls (groups S1, S2 and S4) were significantly
lower than that of unsorted control sperm (P < 0.01). Blastocyst
development at day 7 of the sexed semen groups varied between 3.5% and
28.8% versus 33.6% for non-sexed semen. The individual blastocyst yield
with sexed semen of group S5 (28.8%) was similar to the mean blastocyst
production of the non-sexed control spermatozoa (C, 33.6%; P >
0.05). The remaining five sexed sperm groups resulted in significantly
lower developmental rates of blastocysts on day 7 (S1, 4.9%; S2, 0%;
S3, 0%, S4, 3.5%; S6, 25.8%, P < 0.01). Group S2 showed
microbiological contamination in 50% (four of eight) and S3 in 100% of
the experiments (eight of eight). Progressive motility of sexed sperm
was significantly lower than that of unsorted sperm (S1, 48 +/- 12.0%;
S2, 41 +/- 11.9%; S3, 39.0 +/- 9.9%; S4, 42 +/- 4.6%; P < 0.01; S5,
72 +/- 7.1% and S6, 64 +/- 9.3; P < 0.05 versus C 82 +/- 4.6%). The
percentage of progressive motile spermatozoa showed a good correlation
with the developmental capacity of blastocysts (r(2): >0.70), the
regression parameter was significant (P < 0.01). Furthermore, with a
straw containing 10 x 10(6) sexed spermatozoa significantly lower
number oocytes was fertilized than with the same concentration of
non-sexed sperm (P < 0.01). Our results demonstrate that the
suitability of sperm sorting for in vitro fertilization (IVF) is lower
than no sexed sperm. Our ultrastructural studies showed that
blastocysts produced with flow-cytometrically sex-sorted spermatozoa
possessed deviations in the number and structure of organelles like
mitochondria, rough endoplasmic reticulum (ER) and nuclear envelope.
These morphological alterations may be responsible for compromised
development that observed in embryos produced with sex-sorted
spermatozoa. Thus, we conclude that sperm sex sorting can markedly
affect the efficiency of an IVF-programme.