Recruitment of Pol eta to damaged DNA sites outside S phase

SORIA G, ESSERS J, KANAAR R AND GOTTIFREDI V

Ventura, California, USA

Conferencia; Gordon Research Conference; 2009

Gordon Research Conference

En esta conferencia de alto prestigio internacional la presentación de datos tiene como condición ineludible que dichas observaciones no hayan sido publicadas. Para proteger la confidencialidad de dichos datos la política de estos congresos es la de  no generar libros de resúmenes. El abstract presentado por nuestro grupo se detalla a continuación. Recruitment of Pol eta to Damaged-DNA sites outside S-phase Gast¨®n Soria1, Jeroen Essers2 , Roland Kanaar2 and Vanesa Gottifredi1 1_Cell Cycle and Genomic Stability Laboratory ¨C Fundaci¨®n Leloir . Buenos Aires ¨C Argentina. 2_ Dept. of Genetics ¨C Erasmus MC. Rotterdam ¨C The Netherlands The activity of Pol eta on translesion DNA synthesis (TLS) has been extensively characterized. However, it is not certain if Pol eta function on TLS is restricted to the progressing replication forks or whether it has a delayed activity filling the gaps left behind by replicative polymerases at DNA lesions. Moreover, recent reports suggest that pol eta could be involved in Homologous recombination (HR). However, all the functions of Pol eta described so far are linked to S-phase and no TLS or HR events are expected to take place in the G1 stage of the cell cycle. We have performed a detailed single cell analysis combining the utilization of cell cycle markers and subnuclear damage methods to study the localization of Pol eta across the cell cycle. We concluded that focal recruitment of Pol eta to damaged sites takes place with similar efficiency in both G1/G2 and S-phase cells. Furthermore, we discuss our results regarding the potential connection between the NER and HR machineries and the recruitment of Pol eta outside S-phase.