Crystal structure of the bacteriophytochrome from Xanthomonas campestrispv. campestris: insights into pathogenicityin a light-dependent manner

KLINKE, SEBASTIÁN; OTERO, LISANDRO HORACIO; RINALDI, JIMENA; GOLDBAUM, FERNANDO A.; BONOMI, HERNAN R.

São Paulo

Congreso; First Meeting of the Latin American Crystallographic Association; 2015

Brazilian Crystallographic Association

Phytochromes give rise to the largest photosensor family known to date. However, they are underrepresented in the Protein Data Bank. Plant, cyanobacterial, fungal and bacterial phytochromes share a canonical architecture consisting of an N-terminal photosensory module (PAS2-GAF-PHY domains) and a C-terminal variable output module. The bacterium Xanthomonas campestris pv. campestris, a worldwide agricultural pathogen, codes for a single bacteriophytochrome (XccBphP) that has this canonical architecture, bearing a C-terminal PAS9 domain as the output module. In this talk, we will present the three-dimensional structure of XccBphP determined at 3.25 Å resolution, together with experimental evidence that confirms the participation of this protein in the infectious process and in the modulation of virulence factors.