S-SAD phasing of monoclinic histidine kinase from Brucella abortus at low resolution using true redundancy and multiple crystal data collection (Comunicación Oral)

SEBASTIÁN KLINKE; BEATRIZ G. GUIMARÃES; JIMENA RINALDI; GASTÓN PARIS; FERNANDO A. GOLDBAUM; PIERRE LEGRAND; ANDREW THOMPSON

Campinas

Congreso; Latin American summit meeting on "Biological Crystallography and Complementary Methods"; 2014

IUCr - International Union of Crystallography

The structure of the histidine kinase associated with the light-oxygen-voltage domain from Brucella abortus, containing 968 amino acids in the asymmetric unit, was solved using the off edge anomalous signal from sulfur to a resolution of 2.9 A, by combining data from several isomorphous crystals (spacegroup P21) as well as data collected in multiple orientations of the same crystal (true redundancy). A shorter construct, designed with the aid of the low resolution structure, enabled the production of improved crystals and subsequent refinement of the structure to 2.51 A resolution. The importance of true redundancy in off edge S-SAD will be discussed.