IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
S-SAD phasing on SOLEIL Beamline PROXIMA 1 (Poster)
Autor/es:
P. GOURHANT; BEATRIZ G. GUIMARÃES; TATIANA ISABET; SEBASTIÁN KLINKE; PIERRE LEGRAND; JIMENA RINALDI; ANDREW THOMPSON
Lugar:
Montreal
Reunión:
Congreso; IUCr2014 - 23rd Congress and general assembly; 2014
Institución organizadora:
IUCr2014 - 23rd Congress and general assembly
Resumen:
PROXIMA 1, a beamline for macro-molecular crystallography
at the 3rd generation synchrotron source SOLEIL, is equipped with a
multi-circle goniometer (alpha 50 degrees) as well as a PILATUS 6M
detector. These features, along with the extended energy
range of the beam line towards the low energies (down to 5.5 keV) and
the possibility to adapt the source size to the sample in order to
optimize signal to noise ratio, have made the beam line very attractive
for S-SAD phasing with more than seven examples
of successful de novo phasing achieved over the last two years. The use
of low energies has also proved a significant aid in assisting with
MODEL building. The technical capabilities of the beam line for low
energy data collections will be presented, along
with a number of examples of the successful use of low wavelengths on
the beam line. The importance of combining data from multiple sample
orientations in order to achieve "true multiplicity" will be
highlighted, as well as the importance of combining data
from multiple crystals in order to achieve high multiplicity.
References:
[1] Goulet, A., Vestergaard, G., Felisberto-Rodrigues, C., et al.
Getting the best out of long-wavelength X-rays: de novo chlorine/sulfur
SAD phasing of a structural protein from ATV. Acta Crystallographica D,
2010, 66(3): 304-308