IIBBA 05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
artículos
Título:
Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication
Autor/es:
LUANA DE BORBA; SERGIO M. VILLORDO; N.GABRIEL IGLESIAS; CLAUDIA V. FILOMATORI; LEOPOLDO G. GEBHARD; ANDREA V. GAMARNIK
Revista:
JOURNAL OF VIROLOGY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Lugar: Washington; Año: 2015 vol. 89 p. 3430 - 3437
ISSN:
0022-538X
Resumen:
The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3ยด untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.
