EVALUATION OF THE EFFECTS OF SKIN ULTRAVIOLET LIGHT EXPOSURE ON VACCINATION EFFICACY IN MICE

LEONI, J; CELA, EM; CAMPO, V; GONZALEZ MAGLIO, DH

San Miguel de Tucuman

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Cutaneous exposure to UV radiation (UVr) promotes well-known detrimental effects on health, such as skin cancer development and specific T-cell responses suppression. However, the impact of UV-induced immunosuppression on human vaccination has been poorly studied.Previously, we have reported that a single high UV dose (shUVd - 400 mJ/cm2) promotes skin inflammation while it decreases CHS reaction. In contrast, repetitive low UV doses (rlUVd - 4 consecutive days, 20 mJ/cm2) do not induce inflammation but increase CHS.The aim of the present work was to study the effect of cutaneous exposure to different UV doses on the effectiveness of a tetanus toxoid (TT) and BCG vaccination.C57BL/6 mice were exposed to shUVd or rlUVd and immunized (24h post-UV) with TT (intramuscular) or BCG (intradermal). Non-irradiated vaccinated mice and non-vaccinated mice were used as controls. Specific antibodies were measured by ELISA (TT and BCG) and T cell response was assessed by DTH (only BCG).Specific IgG levels were significantly increased in all TT-vaccinated groups (p