Role of serum and mucosal antibodies in cellular cytotoxicity mechanism during Trichinella spiralis infection in protein deficient rats

FALDUTO GH; BALDI PC; VILA CC; CALCAGNO MA; SARACINO MP; PALLARO AN

Daegu

Congreso; 14th International Congress of Parasitology (ICOPA); 2018

This study was aimed to analyze how protein deficiency affects serum and mucosal antibodies production and their ability to mediate antibody dependent cell mediated cytotoxicity (ADCC) against Trichinella spiralis newborn larvae (NBL).Weaning Wistar rats received protein deficient (PD, 6.5% casein) or control (C, 20% casein) diets. After ten days, both groups were orally infected with T. spiralis muscle larvae (PDI and CI groups, respectively); non-infected rats served as controls (PDNI, CNI). Sera and lung extracts were obtained at different days post-infection (p.i.), and anti-NBL antibodies (IgE, IgG1) were detected by indirect immunofluorescence. In ADCC assays, sera or lung extracts from animals of each group, NBL suspensions and peritoneal cells from CNI rats were co-cultured for 20 h. Results were expressed as NBL mortality percentages (%M) and were analysed using the two-way ANOVA test.Specific antibodies were present since days 6 and 13 p.i. in CI and PDI, respectively. PDI had lower titers of anti-NBL isotypes than CI in both sera (day 13 p.i. IgE: 16 vs. 32-64; IgG1: 32-64 vs. 64-128) and lung extracts (day 13 p.i. IgE: undiluted vs. undiluted-4; IgG1: undiluted vs. 2-4).ADCC was observed using sera from day 13 p.i. in both groups (PDI 21.00±1.41 vs. PDNI 6.61±2.04 %M, P