IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Protein deficiency modifies the gut humoral response to Trichinella spiralis and the fecundity index of adult worms
Autor/es:
VILA, CC; PALLARO, A; FALDUTO, GH; SARACINO, MP; BALDI, PC
Lugar:
Mar del Plata
Reunión:
Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
We have previously demonstrated that protein deficient rats infected with Trichinella spiralis suffer a severe infection with a delayed expulsion of adult worms (AW) from the gut and a higher parasite burden in muscles.Intestinal Trichinella-specific IgE and IgA are known to reduce the fecundity of the AW.The aim of this work was to analyze the effect of a protein deficient diet on the production of intestinal anti-Trichinella antibodies and on AW fecundity index.Weaning Wistar rats received protein deficient (PD, 6.5% casein) or control (C, 20% casein) diets. After ten days, both groups were orally infected with T. spiralis muscle larvae (PDI and CI groups, respectively). Intestine tissue extracts were obtained (PERFEXT method) and specific antibodies against AW excretory/secretory products (AW-ESP) were detected by ELISA. To determine the fecundity index AW were obtained from the intestine of PDI and CI groups and were incubated during 3 h in RPMI medium. Female AW and newborn larvae (NBL) shed by female AW were counted. The fecundity index was calculated as the number of NBL/female AW. Results were analyzed using the two-way ANOVA test.All antibody isotypes against AW-ESP were increased in intestinal extracts of the CI group but only IgG2a had a significant rise in PDI. The PDI group had a higher fecundity index than CI starting at day 9 p.i. (30.58±3.39 vs. 14.32±2.95, NBL/female AW P