Brucella infection of human endometrial stromal cells induces a proinflammatory response

FERNÁNDEZ, ANDREA GISSELLE; FERRERO, MARIANA CRISTINA; ALONSO PAIVA, IVAN MATHIAS; FOCARACCIO, JULIETA VANESA

Mar del plata

Congreso; LXVI reunión anual de la Sociedad Argentina de Inmunología (SAI); 2018

Sociedad Argentina deInmunología

Brucellosis can induce abortion in humans andanimals. Therefore, the interaction between uterine cells and Brucella is important for understandingthe pathogenesis of this disease. Inflammatory phenomena have been shown topromote abortion by other pathogens. In this work, we evaluated theinflammatory response related to Brucella infection of a human endometrialstromal cell line (T-HESC). For this, T-HESC cells were decidualized in-vitro,infected with B. abortus 2308 or B. melitensis H38, or stimulated withconditioned medium (CM) from infected human macrophages, and changes incytokines and chemokines were measured by ELISA. We demonstrate for the firsttime that B. abortus and B. melitensis can infect and proliferate in T-HESC.Expression levels of the decidualization marker prolactin did not change inBrucella-infected T-HESC as compared to non-infected T-HESC (182±13 pg/ml for B. abortus, 176±14 pg/ml for B.melitensis vs. 192± 8 for non-infected cells). The infection induced an increasein interleukin8 (IL-8: 3233± 112 pg/ml for B.abortus, 3907±140 pg/ for ml B.melitensis vs. 1154± 188 pg/ml for non- infected), IL-6 (18 ±2 pg/ml, 17±2.3 pg/ml vs 0± 0 respectively) and monocyte chemoattractant protein 1 (MCP-1:4022±298 pg/ml, 2253±42 pg/ml vs. 1375 ±106 pg/ml respectively) secretion at 48h post- infection. Further, the stimulation of T-HESC for 48 h with CM from B.abortus-infected macrophages induced a significant increase of IL-8 and MCP-1as compared to stimulation with CM from non-infected macrophages (p<0.01).These results suggest that human endometrial stromal cells may provide a localinflammatory environment during Brucellainfection both directly and through interactions with infected phagocytes, potentiallycontributing to the pregnancy complications of brucellosis.