COMPARTAMENTALISATION OF COMMON MUCOSAL IMMUNE SYSTEM BETWEEN GUT AND LUNG IN TRICHINELLOSIS

GENTILINI MV; COHEN M; NUÑEZ GG; VENTURIELLO SM

GRECIA

Congreso; IV HYDRA - MOLECULAR AND CELLULAR BIOLOGY OF HELMINTH PARASITES; 2010

Knowing of importance of the IgE response intestinal in Trichinella spiralis infection and taking into account the concept of compartamentalisation of common mucosal immune system, the aim of the present work was to study the kinetics of the early intestinal immune response in trichinellosis and to compare this to the lung response observed previously during the parasite migration phase at 5-14 days post infection (dpi) (*). METHODS  Peyer Patches (PP), Mesenteric Lymph Nodes (MLN) cell suspensions and lungs and small intestine from Wistar rats infected with T. spiralis were obtained at days 0-6 post-infection (pi). Their cell surface (s) IgE phenotypes were characterized by indirect immunofluorescence. The percentage of fluorescent cells was calculated counting 1000 cells for PP and MLN cells suspension. The number of cells in bronchus associate lymphoid tissue (BALT) and Lamina Propia (LP), small intestinal tissue, were recorded per 15 fields (magnifications 1000x). Both results were obtained through an epiilumination microscope. Total and specific IgE from intestinal and lung extracts were determined by ELISA. CCL28 (MEC) was determined by immunoelectrotransference. RESULTS 1-     X±SE of the percentage and number / 15 fields of sIgE+ cells from PP, MLN, LP and BALT.   DPI % cells No. cells/15 fields    PP                        MLN LP                         BALT 0 19±3a 16±3e 7±2g 88±11l 1 28±0b 16±4 15±3h 108±19 2 37±2c 37±4f 66±13i 135±10m 3 ND ND ND 197±19n 4 29±4 22±6 217±16j ND 6 28±2d 23±5 211±16k 267±5o n=5; p<0.0001: l vs o;  p<0.002: a vs c, l vs n; p<0.01: a vs b, l vs m; p<0.02: g vs h, g vs i, g vs j, g vs k, l vs n; p<0.03 a vs d; p<0.1: l vs m. Student`s t test.   2-     Total and specific IgE anti-parasite in lung and intestinal extract were detectable from 1 dpi. 3-     CCL28 (MEC) was present at 1-3 dpi.   CONCLUSION 1-sIgE+ cells appear early in PP and LP and then migrate to MLN and BALT since 2 dpi after T. spiralis infection. 2-This cell flow seems to be led by the presence of CCL28 (MEC). 3-Our model emphasizes the concept of common mucosal immune system providing immune reactivity not only in the induction site (intestine) but also in distal sites suc as BALT, where the lung is likely to have a protection role against the parasite migration stage.