OMP16 COADMINISTERED AS A MUCOSAL ADJUVANT INDUCES A TH1 IMMUNE RESPONSE

IBAÑEZ AE; CORIA LM; PASQUEVICH KARINA; GIAMBARTOLOMEI GUILLERMO; CASSATARO JULIANA

Viña del Mar. Chile

Congreso; 9th Latin American Congress of Immunology; 2009

ALAI

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Previous results demonstrated that the protein moiety of the Brucella Omp16 lipoprotein (Omp16S) administered by the oral route has self-adjuvanting properties. This prompts us to study its mucosal adjuvant capacity using as a model antigen ovalbumin (OVA) by two different mucosal routes: oral and nasal. For this purpose mice were immunized orally with OVA, Omp16S+OVA, or choleric toxin (CT)+OVA. By flow cytometry we determined that the percentage of CD8+ T cells expressing α4β7 integrin increased in mesenteric lymph nodes from Omp16S+OVA (4.53%) and CT+OVA (16.43%) in comparison with OVA (1.64%) immunized group. We also observed a significant (P<0.01) delayed type hypersensitivity (DTH) response to OVA injection in Omp16S+OVA and CT+OVA while OVA group did not. Similarly, splenocytes from mice immunized intranasally with Omp16S+OVA or CT+OVA secreted IFN-γ but not IL-4 in response to OVA stimulation in vitro. By flow cytometry we determined that the percentage of CD4+ and CD8+ T cells producing intracellular IFN-γ increased in Omp16S+OVA (0.97%; 1.08%) in comparison with CT+OVA (0.28%; 0.22%) and OVA (0.23%; 0.47%). In conclusion, Omp16S as mucosal adjuvant induce the migration of CD8+ T cells to gastrointestinal mucosa, T cells responses in vivo and IFN-γ production by CD4+ and CD8+ T cells.