Role of serum antibodies from protein deficient rats in antibody dependent cellular cytotoxicity mechanism against newborn larvae of Trichinella spiralis.

FALDUTO G.H.; BALDI P.C.; SARACINO M.P.; PALLARO A.; VILA C.C.; CALCAGNO M.A.

Buenos Aires

Congreso; Reunión conjunta de sociedades de Biociencias: SAIC, SAIB, SAI, SAA, SAB, SAB, SAFE, SAFIS, SAH, SAP.; 2017

ROLE OF SERUM ANTIBODIES FROM PROTEIN DEFICIENT RATS IN ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY MECHANISM AGAINST NEWBORN LARVAE OF TRICHINELLA SPIRALISVila Cecilia Celeste1*, Saracino María Priscila1, Falduto Guido Hernán1, Calcagno Marcela Adriana1, Pallaro Anabel Nora2, Baldi Pablo César1.1Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología-IDEHU (UBA-CONICET), Buenos Aires, Argentina.2Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Nutrición, Buenos Aires, Argentina.*PresentingauthorAbstract: This study was aimed to analyze how protein deficiency affects serum antibodies production and their ability to mediate antibody dependent cellular cytotoxicity (ADCC) against T. spiralis migrant stage (newborn larvae, NBL).Weaning Wistar rats (n=10/group) received protein deficient (PD, 6.5% casein) or control diets (C, 20% casein). After ten days, both groups were orally infected with T. spiralis muscle larvae (PDI, CI); non-infected rats served as controls (PDNI, CNI). Sera were obtained on days 6, 13 and 33 post-infection (p.i.), and effector antibodies against NBL (IgE, IgG1) were detected by indirect immunofluorescence. In ADCC assays, NBL suspensions, sera from animals of each group and peritoneal cells from CNI rats were co-incubated for 20 h. Results were expressed as NBL mortality percentages (M%) and were analyzed using the two-way ANOVA test.Specific antibodies were present since days 6 and 13 p.i. in CI and PDI, respectively. PDI had lower titers of anti-NBL isotypes than CI on days 13 (IgE: 16 vs. 32-64; IgG1: 32-64 vs. 64-128) and 33 p.i. (IgE: 32-64 vs. 256; IgG1: 1024 vs. 2048). As in the CI group, ADCC was observed in the PDI group when sera from days 13 (M%: PDI 21.00±1.41 vs. PDNI 6.61±2.04 p