Proinflammatory response of human endometrial epithelial cells to Brucella abortus infection

FERNANDEZ, AG; ALONSO PAIVA, I; FERRERO, MC; MUÑOZ GONZALEZ, F; BALDI, PC; RITTER, A; FOSSATI, CA

Buenos Aires

Congreso; II Reunión Conjunta de Sociedades de Biociencias y LXV Reunión Anual de la Sociedad Argentina de Inmunología; 2017

Sociedad Argentina de Inmunología

Brucella induces abortion in humans and animals.Therefore, the interaction between uterine cells and Brucella is important for understanding the pathogenesis of this disease.In this work, we evaluate the inflammatory response (chemokine and metalloproteinases) and the expression of hormonal receptors and various microRNA related to infectionor inflammation processes in thehuman endometrial epithelial cell line Ishikawa,after infectionwith B. abortus2308 or stimulation with conditioned medium (CM) from infected human phagocytes or trophoblasts. The Ishikawa cells, the monocyticcell line THP-1, the trophoblastic cell line Swan-71 andmonocytes and neutrophilsisolated from peripheral blood were infected with B. abortus for 2 h. After treatment with gentamicin, supernatants were collected at 24 and/or 48 h. Here we show that B.abortus invades and replicates in Ishikawa cells without inducing an increasein interleukin 8 (IL-8) or monocyte chemoattractant protein 1 (MCP-1) secretion. However, the stimulation of Ishikawa cells for 48 hwith CM from B. abortus-infected THP-1 cells,primary monocytes or Swan-71 cells induces a significant increase of IL-8 and MCP-1as compared to stimulation with CM from non-infected cells (p