Lung immune response during intestinal infection with Trichinella spiralis

GENTILINI MV, NUÑEZ GG, NUÑEZ M, VENTURIELLO SM

Tahoe City, California. UEA

Simposio; Pathogenesis and Immune Regulation in Helminths Infections; 2009

We have demonstrated that the intestinal infection by T. spiralis evokes an Th2-type inflammatory response in the lung.   Our aim was to determine the cytokines (Cks), the chemokine CCL11 and the immunoglobulins (Igs) response in the lung parenchyma of Wistar rats at 1, 2, 3, 6 and 13 days post-infection (dpi). Lungs were obtained (n:5/day) and subjected to the Perfext method to be used in the ELISAs. The ELISPOT was used to assess the direct secretion of Igs. Anti-newborn larvae (NBL) Igs were determined by IFA. Results showed that: a) Animals presented specific IgA from the day 1 pi on, being a 100% positive by day 3 pi. b) Specific IgG1 was positive from day 1 pi in a 50% of the animals, being all of them positive by day 6 pi. c) Specific IgG2a was positive in all the animals from day 1 pi and the positivity percentage decreased over time, finding no positive animals by day 13 pi. d) Specific IgE was detected from the day 1 pi in a 50% of the animals, reaching 100% from day 6 on. e) The ELISPOT assay demonstrated the secretion of specific IgA (3 dpi) and IgE (6 dpi). f) IFNƒ× and TNFƒÑ were increased from day 1 pi, IL-4 and IL-10 were increased from day 2 to 13 pi. High levels of CCL11 were increased throughout the time span studied. g) IgE anti-NBL was found on day 13 pi.   It can be suggested that early mechanisms of both the innate and adaptative immunity are triggered from the gut exerting a rapid allergic response in the lungs that together with the IgE anti-NBL would play a role in the effector defense mechanism.