IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Anti-Inflammatory effect of Smilax campestris rhizomes aqueous extract: Inhibition of NF-κB nuclear translocation, pro-inflammatory cytokines, MMP-9 and superoxide anion levels in THP-1 human macrophages.
Autor/es:
CANELLADA A.; RUGNA A ; SALAVERRY LS; REY-ROLDÁN E; GENTILE T; PARRADO AC; BLANCO G, ; MANGONE F
Reunión:
Jornada; I Jornadas del Departamento de Microbiología, Inmunología, Biotecnología y Genética; 2018
Institución organizadora:
Departamento de Inmunología, Microbiología y Biotecnología, FFyB, UBA
Resumen:
Macrophages play a crucial role in inflammatory responses. Activated macrophages produce several mediators of inflammation that regulate the immune response. Extracts of S. campestris have been used in folk medicine in the treatment of several inflammatory diseases, however the cellular and molecular mechanisms involved in the effects observed are still unknown. In this study, we aimed to study anti-inflammatory effect of S. campestris aqueous extract in THP-1 PMA- differentiated macrophages. The latter cells were incubated with different concentrations of S. campestris aqueous extract (10, 100, 1000 and 10000 ng of tannic acid/mL extract) and then activated or not with lipopolysaccharide (LPS, 1 μg/mL). The pro- inflammatory cytokine levels (ELISA), cell cytotoxicity (LDH release), metalloprotease activity (zymography), superoxide anion and glutathione levels (flow cytometry) and NF-κB/ I-κB expression (WB) were assayed. A p value