SAgs of the egc operon: Impact on the innate immune response

FERNÁNDEZ MARISA MARIEL; MALCHIODI EMILIO LUIS; IANNANTUONO LÓPEZ, LAURA; SARRATEA, BELÉN; NOLI TRUANT, SOFIA; JANCIC, CAROLINA; ANTONOGLOU, BELÉN

CABA

Congreso; IV International Congress in Translational Medicine.; 2018

Bacterial superantigens (SAgs) are enterotoxins that bind to MHC-II and TCR, activating as much as 20% of T cells and promoting a cytokine storm which enhances endotoxic shock and produces immunosuppression, hindering the immune response against bacterial infection. In this work, we investigated the effect on the innate immune response of four natural variants of SAgs (SEG, SEI, SEO and SEM), encoded by the egc operon in autochthonous S. aureus strains.First, we analyzed the effect of SAgs on monocytic THP-1 cells finding a strong inhibition of the proliferation determined by 3H-thymidine. Thus, 100 μg/ml of SEO, SEG and SEI inhibited proliferation up to 70% while SEM inhibited ~30% (p