Effect of SAgs of the egc operon on innate immune cells.

EMILIO L. MALCHIODI; JANCIC, CAROLINA; SARRATEA, BELÉN; ANTONOGLOU, BELÉN; NOLI TRUANT, SOFIA; MARISA M. FERNANDEZ; IANNANTUONO LÓPEZ, LAURA; TOWSTYKA, NADIA

CABA

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica (SAIC), Sociedad Argentina Bioquímica y Biología Molecular (SAIB), Sociedad Argentina Inmunología (SAI), Sociedad Argentina Fisiología (SAFIS), Sociedad Argentina Farmacología Experimental (SAFE), Sociedad Argen

Bacterial superantigens (SAgs) are enterotoxins that bind to MHC-II and TCR molecules, activating as much as 20% of T cells and promoting a cytokine storm which enhances endotoxic shock and produces immunosuppression, hindering the immune response against bacterial infection. The egc operon reported in autochthonous S. aureus strains includes natural variants of the SAgs genes seg,sei, sem, sen and seo. We have previously characterized the complementary activityof SEG, SEI, SEO and SEM on phagocytic cells, and reported a M1 responsetendency.In the present work, we investigate if other cells of the innate immunity are responsible for this proinflammatory profile. Thus, we evaluated the SAgs effect on human purified γδ T cells from healthy donors by flow cytometry and ELISA. We found that, in contrast to the effect on αβ T cells, SAgs activated γδ T cells from 0.1μM (p