The Study of a polymeric adjuvant (Montanide Gel 01) in a vaccine against Bovine Respiratory Disease.

DÍAZ A.; CASTRO M; ALMOZNI B.; CANELLADA A; MANGONE F

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias.; 2017

Sociedad Argentina Inmunología

The Bovine Respiratory Disease (BRD) is a multi-factorial disease that causes high morbidity and mortality in cattle. The disease involves a sequential cascade of events initiated by stress; thus, predisposing animals to viral infections which facilitate rapid bacterial invasion from the upper respiratory tract to lungs. BRD etiology is composed by various viruses and several bacteria: Mannheimia haemolytica (MH), Pasteurella multocida (PM), Histophilus somni, and Mycoplasma bovis. Although BRD vaccines are worldwide produced and applied, there is a need of new therapeutic-adjuvant strategies in order to minimize the economic impact of respiratory syndromes.Montanide Gel is a polymeric adjuvant, based on a dispersion of stable gel particles, which enhance the immune response related to a depot effect with slow-release of the antigens.This research aims to study the action of a newer adjuvant, Montanide Gel 01 (Seppic), employing MH and PM as antigen in a murine model. BALB/c mice were subcutaneously immunized with a commercial vaccine and various experimental vaccines in three doses. Inactivated MH or PM were formulated with Montanide Gel at 5% or 10%, or with aluminum hydroxide (AH). Mice received the vaccine on days 0, 15 and 27. Blood samples were obtained on days 0, 10, 27 and 38. Control groups received the adyuvants with PBS. Specific antibody levels IgG, IgG1, and IgG2a anti-PM and anti-MH were measured by in house indirect ELISA.Montanide vaccine shows significative differences in the production of IgG, IgG1 and IgG2a anti MH compared with AH vaccine (p< 0,0001; p< 0,001; p< 0,01). In relation with IgG and IgG1 anti PM, antibody levels are higher than the commercial vaccine, although there are no significative differences. On the other hand, there are significative differences in IgG2a between the vaccines (p< 0,001).To conclude, we can say that Montanide Gel vaccine induced higher antibody levels than commercial and AH vaccine, so it could be a possible adyuvant candidate to use with cattle. Further studies will be performed analyzing this vaccine in cattle.