IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hyaluronan levels in bronchoalveolar lavage of mice with induced allergic asthma
Autor/es:
S. E. HAJOS; G. M. ERNST; R. C. RUSSO; S. LOMPARDIA; E. M. C. ALVAREZ
Lugar:
Kobe, Japon
Reunión:
Congreso; 14th International Congress of Inmunology; 2010
Resumen:
Hyaluronan (HA) is an important constituent of the extracellular matrix, which accumulates during inflammatory lung disease like asthma. Low molecular weight (LMW-HA) but not high molecular weight (HMW-HA) has shown to induce inflammatory cytokines secretion. CD44 is the principal receptor. The aim of this work was to analyze the roll of HA-CD44 interaction in asthma induced inflammation. For this purpose, we establish a model of asthma by intranasal inoculation of dermatophagoides pteronyssinus (Dp) in BALB/c mice, which produced a significant increase of eosinophils in bronchoalveolar lavage (BAL). Histochemical analysis showed that lung of mice sensitized with Dp presented lymphoid infiltration associated to bronchial tissue, while control mice presented a normal structure. Flow cytometry analysis showed that the percentage of CD44+ cells in BAL was significantly increased in Dp treated mice compared to non treated ones (47.4±3.6 vs. 14.0±7.1%, p<0.05). HA levels in BAL were measured by ELISA finding higher HA concentration in Dp treated animals compared to controls (5.68±1.58 vs. 0.51±0.11ug/ml, p<0.05) while Dp mice pre-treated with either Budesonide or Dexametasone showed decreased HA levels (0.42±0.07 and 0.39±0.12 vs. 5.68±1.58ug/ml, p<0.05 respectively). Migratory capacity of cells from BAL was also evaluated showing that Dp sensitized mice present higher migration towards LMW-HA as compared to RPMI (migration index 2.2±0.2 vs. 1) but not towards HMW-HA. When BAL cells were pre-incubated with anti-CD44 antibody, migration towards LMW-HA was inhibited (migration index 1.1±0.2). We conclude that HA-CD44 interaction is involved in inflammation induced by allergic asthma emphasizing its importance for therapy.