Kinetic appearence in lung and serum of IgE and IgA in Trichinella spiralis infected rats

GENTILINI V., NUÑEZ GG, VENTURIELLO SM

París

Congreso; Xth European multicolloquium of Parasitology (EMOP),; 2008

Kinetics of appearance of IgE and IgA in lungs and sera of Trichinella spiralis-infected rats. Considering the importance of IgA and IgE at mucosal levels and their participation in effector mechanisms against helminths, the aim of this study was to compare the kinetics of appearance of total and specific IgA and IgE in lungs vs serum during Trichinella spiralis infection in rats. To this end, sera and lung extracts (PERFEXT) were obtained from infected (n:5) and control (n:5) SPF Wistar rats 3, 6 and 13 days post-infection (dpi). Immunoglobulins levels were determined by indirect (antigen: excretory-secretory products, ESP) and capture ELISA. Histochemical and immunohistochemical techniques were employed to analyze paraffin-embedded lung sections. IgE and IgA were detected from 3 dpi on in both lungs and sera reaching maximum values on 13 dpi. Anti-ESP IgA was detected in lungs in 4/5 animals and in sera in 5/5 animals. Anti-ESP IgE was also detected in lungs in 4/5 animals and in sera in 1/5 animals. In lung sections, an inflammatory response was observed with presence of CD4+ and IgE+ cells from 3 dpi on. Results showed a similar kinetics of appearance of IgA and IgE in lungs and sera, where the early appearance of anti-ESP IgE in lungs and anti-ESP IgA in sera in a greater number of animals was noteworthy. Results demonstrate the participation of the lung in the early immune response during this parasitic infection and suggest the capacity of T. spiralis to induce an immune response in mucosal sites far apart from the invasion sites.