THE AQUEOUS EXTRACT OF RHIZOMES OF AN ARGENTINIAN MEDICINAL PLANT (SMILAX CAMPESTRIS) DECREASES PROINFLAMMATORY CYTOKINES PRODUCTION IN HUMAN LPS-ACTIVATED MACROPHAGES.

MANGONE F; GENTILE, MARÍA TERESA; PARRADO CECILIA; BLANCO G; REY-ROLDÁN, ESTELA; SALAVERRY, LUCIANA; RUGNA, ANA; CANELLADA, A

Mar del Plata

Congreso; LXIV ANNUAL MEETING ARGENTINE SOCIETY OF IMMUNOLOGY (SAI), LXI ANNUAL MEETING ARGENTINE SOCIETY FOR CLINICAL INVESTIGATION (SAIC); 2016

Folk medicine has used different plant extracts for their anti-inflammatory and antioxidant effects. Smilax campestris (S.campestris) or ?sarsaparilla? is a medicinal plant wildly distributedin northern Argentina. Previously we showed an antiinflammatoryeffect of aqueous extract of rhizomes of S. campestris onmetalloproteinase-9 activity (MMP-9), an enzyme involved in theextracellular matrix remodeling process. S. campestris significantlydecreased the activity of MMP-9 in LPS-activated macrophagecells. In the current study, we evaluated the effect of this extracton proinflammatory cytokines (IL-1beta, IL-6), the chemokine IL-8levels (ELISA) and cytotoxicity (lactate dehydrogenase releaseassay, LDH) in human monocyte-macrophage cells, THP-1. Cellswere stimulated under two conditions: the absence (basal state) orpresence (activated state) of the inflammatory stimulus, lipopolysaccharide(LPS). Cells were incubated with different concentrationsof S. campestris extract for 24/48 hs (10, 100, 1000, 10.000ng of tannic acid/ml extract); LPS-activated macrophage cells wereaditionally incubated with LPS (1 ug/ml) to induce an inflammatorycontext. The aqueous extract of rhizomes of S. campestris did notalter IL-1beta, IL-6 and IL-8 levels in basal conditions (absenceof LPS). But interestingly, the extract significantly decreased allcytokines meassured levels in human activated macrophagecells (p