EXTRACTS OF AN ARGENTINIAN PLANT USED IN ANTIRHEUMATIC FOLK MEDICINE INHIBITED NFAT NUCLEAR TRANSLOCATION IN MURINE MACROPHAGES, ACTING AT OESTROGEN RECEPTORS ALPHA.

MANGONE F; DÍAZ AM; REY-ROLDÁN, ESTELA; SOTELO A; GENTILE, MARÍA TERESA; CANELLADA, A; SALAVERRY, LUCIANA; RUGNA, ANA; CASTRO MS

Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2016

Smilax campestris Griseb is widely distributed in the northof Argentine and it is used as antirheumatic folk medicine. Wepreviously investigated the effect of aqueous extracts of smilax(SM) on the osteoclast differentiation of murine macrophages.We found that SM diminished RANKL (RL)-induced osteoclastlikecells, cathepsin K mRNA expression and MMP activity, in adose dependent manner, without affecting RAW 264.7 cell proliferation.RL-induced osteoclastogenesis involves the activation ofNFAT signaling in macrophages. It has been described that plantextracts inhibit osteoclastogenesis acting at oestrogen receptors(ER). The aim of the current study was: 1) To determine whetherSM inhibit the activation of NFAT in RAW cells; 2) To investigatewhether the SM inhibition involved ER. We also analyzed the effectof SM on murine bone marrow derived osteoclast precursors(BMD OC). RAW cells were cultured 16 h with SM (10-100-1000ng/ml) or estradiol (E2, 10-7; 10-8 M) and then cultured 1 h withphorbol myristate acetate plus calcium ionophore (PIo) to inducethe nuclear traslocation of NFAT. To analyze ER involvement, cellswere incubated 1 h with ERa or ERb antagonist (1 and 5 uM) prio rto SM treatment. We measured NFAT translocation by westernblot. BMD OC were obtained by incubating BMD mononuclearcells during 7 days with RL plus MCSF in the presence or not ofSM. MMPs secretion to culture supernatants was assessed byzymography. Treatment of RAW cells with SM or E2 diminishedthe NFAT translocation in a dose dependent manner. Inhibitionof 90.1±4.5% (p