Alterations in pulmonary function during Trichinella spiralis infection

ACOSTA P; VILA CC; VENTURIELLO SM; FALDUTO GH; POLACK F

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; 2016

Experimental parasitic infection caused by Trichinella spiralis provokes, in the murine model, an early allergic inflammatory response in the lung, before and during the migratory stage (MS) passage through this organ. The present study aimed to analyze whether this allergic response provokes changes in pulmonary function and related parameters at days 1, 2, 3, 6, 9, 13, 30 post-infection (p.i.).Pulmonary function was assessed by measuring pulmonary resistance (R) at increasing doses of methacholine (cholinergic bronchoconstrictive agent). Lung parenchyma mast cells presence and degranulation were analyzed by: histological stains (toluidine Blue), histamine detection in bronchoalveolar-lavage (BAL; ELISA), presence of CD45+ FcepsilonRI+ lung cells (flow cytometry) and anti-MS IgE in BAL, lung tissue extracts and sera (immunofluorescence assay). Data were analyzed using the one-way ANOVA test.Our results revealed: I- an increased R at days p.i. studied, compared to non-infected animals (p