IDEHU   05542
INSTITUTO DE ESTUDIOS DE LA INMUNIDAD HUMORAL PROF. RICARDO A. MARGNI
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Extracts of an argentinian plant used in antirheumatic folk medicine inhibited NFAT nuclear translocation in murine macrophages acting at oestrogen receptors alpha. .
Autor/es:
MANGONE F; SALAVERRY, LUCIANA; SOTELO A; DÍAZ AM; RUGNA, ANA; GENTILE, MARÍA TERESA; REY, ESTELA; CASTRO MS; CANELLADA, A
Reunión:
Congreso; Reunión Científica conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), ociedad Argentina de Inmunología (SAI), Sociedad Argentina de Farmacología Experimental (SAFE), AACYTAL; 2016
Resumen:
Folk medicine has used different plant extracts for their anti-inflammatory and antioxidant effects. Smilax campestris (S. campestris) or "sarsaparilla" is a medicinal plant wildly distributed in northern Argentina. Previously we showed an antiinflammatory effect of aqueous extract of rhizomes of S. campestris on metalloproteinase-9 activity (MMP-9), an enzyme involved in the extracellular matrix remodeling process. S. campestris significantly decreased the activity of MMP-9 in LPS-activated macrophage cells. In the current study, we evaluated the effect of this extract on proinflammatory cytokines (IL-1beta, IL-6), the chemokine IL-8 levels (ELISA) and cytotoxicity (lactate dehydrogenase release assay, LDH) in human monocyte-macrophage cells, THP-1. Cells were stimulated under two conditions: the absence (basal state) or presence (activated state) of the inflammatory stimulus, lipopolysaccharide (LPS). Cells were incubated with different concentrations of S. campestris extract for 24/48 hs (10, 100, 1000, 10.000 ng of tannic acid/ml extract); LPS-activated macrophage cells were aditionally incubated with LPS (1 ug/ml) to induce an inflammatory context. The aqueous extract of rhizomes of S. campestris did not alter IL-1beta, IL-6 and IL-8 levels in basal conditions (absence of LPS). But interestingly, the extract significantly decreased all cytokines meassured levels in human activated macrophage cells (p